Volume 8 Issue 2 ( June 2021 )


(E)-N-(3,4-dimethylisoxazol-5-yl)-4-((4-hydroxy-2-oxo-2H-chromen-3-yl) diazenyl)Benzenesulfonamide; A New Synthetic Anti-Herpetic Agent for HSV-1

Ashish Kumar, Brij Kishore Tiwari, Mamta Mishra, Navneet Kumar


(E)-N-(3,4-dimethylisoxazol-5-yl)-4-((4-hydroxy-2-oxo-2H-chromen-3-yl)diazenyl)benzene sulfonamide (HDF) was prepared by condensation of 4-hydroxy coumarin and 4-amino-N-(3,4-dimethylisoxazol-5-yl)benzene sulfonamide (sulphafurazole). The structure of synthesized compound was established using IR, 1H and 13C- NMR spectral analysis. On evaluation of in-vitro anti-herpetic activity of this compound against HSV-1, compound was found able to inhibit viral replication at late stages of infection by inhibiting the replication of HSV- 1. This anti herpetic nature of HDF was evidenced by the reduction of specific gene productsβ-(ICP-6) and γ-(ICP-5 and gB) groups in western blotting.

Keywords: coumarin-sulphonamide, 4-hydroxy coumarin, HSV-1, immunoblot analysis, post infection