To develop the non-nucleoside antiviral agent for genital herpes caused by HSV-2, (Z)-4-((7-amino-4-methyl-2-oxo-2H-chromen-3-yl)diazenyl)-N-(5,6-dimethoxypyrimidin-4-yl)benzenesulfonamide (ADPB) was synthesised by reaction of 6-amino-4-methyl-2H-Chromen-2-one and 4-amino-N-(5,6-di-methoxypyrimidinyl)benzene sulphonamide. The structure of ADPB (molar mass 496) was established using Infrared, 1H and 13C-NMR spectroscopy. During the assessment of in-vitro anti-herpetic activity of ADPB against HSV-2, this coumarin sulphonamide derivative was found as potent antiviral agent for HSV-2 indicated by its selectivity index greater than 10 and less cytotoxicity. The potential of compound to inhibit the replication of virus at post-infection was visualized by reduction of specific gene products[ β-(ICP-6) and γ-(ICP-5 and gB) groups] in western blotting.
Keywords: Antiviral agent, genital herpes, Acyclovir, western blot analysis, coumarin-sulphonamide