EVERGREEN

Joint Journal of Novel Carbon Resource Sciences and Green Asia Strategy

ISSN:2189-0420 (Print until Mar 2020)
ISSN:2432-5953 (Online)

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Sorghum Juice from the 'Super 2' Variety: A Promising Carbon Source for Oleaginous Yeast in Biodiesel Production

Mauritz Nicolaas Wattimena1, Toga Pangihotan Napitupulu2, Atit Kanti3, Idris Idris2, Leonard Wijaya4, Miftahul Ilmi5, Wijanarka Wijanarka1, Fatmawati Fatmawati6, I Made Sudiana2,*
1Department of Biology, Diponegoro University, Indonesia
2Research Center for Applied Microbiology, National Research and Innovation Agency, Indonesia
3Research Center for Biosystematics and Evolution, National Research and Innovation Agency, Indonesia
4Research Center for Environmental and Clean Technology, National Research and Innovation Agency, Indonesia
5Faculty of Biology, Universitas Gadjah Mada, Indonesia
6Research Center for Food Crops, National Research and Innovation Agency, Indonesia
*Author to whom correspondence should be addressed:
E-mail: i.made.sudiana.example@university.edu (IMS)
Evergreen, Vol. 12, Iss. 02, pp. 689–700, 2025
DOI: 10.5109/7363468
Creative Commons Attribution 4.0 International Creative Commons Attribution 4.0 International
Received: April 29, 2024 | Revised: February 05, 2025 | Accepted: April 01, 2025 | Published: June 2025
Abstract
This study investigates cost-effective substrates for yeast-driven biodiesel production, focusing on sweet sorghum ‘Super 2’ juice. Lipomyces starkeyi InaCC Y-285, Candida orthopsilosis InaCC Y-302, and Candida oleophila InaCC Y-306 were cultured with adjusted carbon concentrations. Glucose 4%+Sorghum Juice medium significantly increased biomass to 8.03, 8.64, and 6.94 g.L-1 and lipid content by 26.60, 33.77, and 48.72% compared to Glucose 12% medium. These strains predominantly produced oleic and stearic acids in this medium, emphasizing Glucose 4%+Sorghum Juice’s potential for efficient biodiesel production by oleaginous yeasts.
Keywords
yeasts ; sorghum ; fatty acid composition ; carbon source ; oleaginous yeast ; lipid accumulation
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